Fundamental laboratory approaches for biochemistry and biotechnology

Başlık:

Yazar:

Ninfa, Alexander J.

ISBN:

9781891786006

Ek Yazar:

Ninfa, Alexander J.

Yayım Bilgisi:

Fiziksel Tanım:

xi, 372 pages : illustrations ; 30 cm.

Contents:

Basic Practices and Techniques in the Biochemistry Laboratory -- Chp. 2. Spectrophotometry -- Chp. 3. Quantification of Protein -- Chp. 4. Chromatography -- Chp. 5. Gel Electrophoresis of Proteins -- Chp. 6. Overview of Protein Purification -- Chp. 7. Isolation and Characterization of the Enzyme Alkaline Phosphatase From Escherichia Coli -- Chp. 8. Enzyme Kinetics -- Chp. 9. Enzymatic Methods of Analysis -- Chp. 10. Ligand Binding -- Chp. 11. Recombinant DNA Techniques -- Chp. 12. Polymerase Chain Reaction (PCR) Technology -- Chp. 13. Using The Personal Computer and The Internet For Biochemical Research.

Konu Terimleri:

Biyokimya -- Laboratuar kılavuzları.

Biochemistry -- Laboratory manuals.

Biyoteknoloji -- Laboratuar kılavuzları.

Biotechnology -- Laboratory manuals.

Moleküler biyoloji -- Laboratuar kılavuzları.

Molecular biology -- Laboratory manuals.

Added Author:

Ballou, David P.

Mevcut:*

Library	Materyal Türü	Barkod	Yer Numarası	Durum
Searching... Pamukkale Merkez Kütüphanesi	Kitap	0017444	QP519 .N55 1998	Searching... Unknown

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Özet

An excellent biochemistry laboratory text for advanced undergraduate and first year graduate students in biochemistry and other life sciences, this text provides a logical framework for training students how to approach research problems and conduct and evaluate scientific research. Each chapter provides extensive background on the principles underlying methods used to research, followed by experiments designed to illustrate those principles.

Author Notes

Alexander J. Ninfa is Associate Professor in the Department of Biological Chemistry at the University of Michigan and is internationally recognized for his work on the biochemical mechanisms of signal transduction and transcriptional regulation in bacteria.

David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.

Preface.

Chapter 1 Basic Practices and Techniques in the Biochemistry Laboratory

Chapter 2 Spectrophotometry

Chapter 3 Quantification of Protein Concentration

Chapter 4 Chromatography

Chapter 5 Gel Electrophoresis of Proteins

Chapter 6 Overview of Protein Purification

Chapter 7 Isolation and Characterization of the Enzyme Alkaline Phosphatase From Escherichia Coli

Chapter 8 Enzyme Kinetics

Chapter 9 Enzymatic Methods of Analysis

Chapter 10 Ligand Binding

Chapter 11 Recombinant DNA Techniques

Chapter 12 Polymerase Chain Reaction (PCR) Technology

Chapter 13 Using the Personal Computer and the Internet for Biochemical Research

Appendix 1 Safety Checklist

Appendix 2 An Example of an MSDS

Index

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Özet

Özet

Author Notes

Table of Contents